With the EPA finalizing legally enforceable Maximum Contaminant Levels (MCL) for multiple PFAS compounds in drinking water at low ng/L concentrations, it is crucial to understand how different PFAS analytes in reference materials react to solvents and surface interactions. Using the Advanced Materials Technology HALO 90 Å PFAS 2.7µm x 2.1mm x 100mm column, we explored parameters that could impact data integrity with the analytes in EPA Method 1633, UCMR 5 and EU Drinking Water Directive. The method was developed on ThermoFisher Scientific Vanquish UPLC with Exploris 120 HRAMS, emphasizing the influence of solvent on sample stability and surface effects. Various solvents, blends, and modifiers were evaluated, along with investigating interactions between the reference material ampule and sample vial surfaces and PFAS response factors for designing optimal reference materials. Ratioing response factors against concentrations helped determine suitable concentrations that give similar responses. After the individual analytes were optimized, the response factors were ratioed against the concentrations to give the appropriate concentration for similar response factors.
Find out more in our latest poster from our experts, Daniel Biggerstaff PhD (End-Market Sales Technical Director) and HuiChen W. Stravos PhD (Research Scientist).
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PFAS Reference Material Optimisation on LC – High Resolution Accurate Mass Spectrometry