BHQnova Probes: Double-quenched qPCR probes for high-sensitivity applications
BHQnova probes are a newly developed probe format with quenching efficiency that provides improved signal-to-noise ratio over standard dual-labeled hydrolysis probes.
App Note: Improved Function with Double-Quenched BHQ® Probes for qPCR
Excerpt -- A sensible approach to improve quenching efficiency is to preserve the terminal quencher for its blocking function while incorporating a second quencher at an internal position. That is the basis of novel double-quenched probe-type from LGC Biosearch Technologies, called BHQnova™ probes:
5’ - [FAM]- Oligo sequence1 - [Nova] - Oligo sequence2 - [BHQ-1] - 3’
Standard Hydrolysis Probe vs BHQnova Probes
Figure 1. qPCR amplification curves for BHQnova probes (red) demonstrate the improved quenching and S:N of BHQnova probe vs. standard hydrolysis probe (black) of the same sequence.
App Note: Improved qPCR Assays for Copy Number Variation Using Double-Quenched BHQ® Probes.
Excerpt 1 -- In this study, we designed a BHQnova probe to CYP2D6, a common human CNV target, using LGC Biosearch Technologies’ RealTimeDesign™ software. This region of the human genome is known to harbor sequence rearrangements with clinical significance, through variation in gene copy number. Similarly, we apply BHQnova™ probes for analysis of wheat samples that contain copy number variations that influence crop characteristics. The results reveal accurate quantification, but also the performance advantages of double-quenched probes.
Improved Signal-to-Noise Values in CYP2D6 Assay
Get started with a FAM BHQnova Probe for $70!
Use the table below to help choose the option which is right for you. All probes are RP HPLC purified. Size refers to final delivered amount.
| BHQnova Probe Selection | ||||
| Fluorophore | XS: 5 nmol | S: 10 nmol | M: 25 nmol | L: 60 nmol |
| FAM | $70 at 2 nmol delivered | $165 | $270 | $405 |
| CAL Fluor® Orange 560 | $150 | $225 | $310 | $470 |
| CAL Fluor Gold 540 | $150 | $225 | $310 | $470 |
| HEX | $150 | $235 | $340 | $505 |
| TET | $150 | $235 | $340 | $505 |
Pick the recommended fluorophore for your instrument by checking out this interactive spectral overlay tool.