Alternative Minimum Inhibitory Concentration (MIC) Test Methods: The use of Optical Density Readings to Determine MIC Endpoint
Minimum inhibitory concentrations (MIC)/ in vitro susceptibility of bacteria to antimicrobials are determined by examining 96-well microtitre plates for visible growth. One scientist reads the plates by eye and a second scientist checks that they are in agreement. This is time consuming, involving two scientists. The results are open to interpretation to a certain degree between the scientists and therefore both scientists must be adequately trained to read plates in order to obtain reproducible results. As an alternative method, optical density (OD) readings have been used to determine the MIC endpoint in a broth microdilution test.
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